Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany /
Electrofocusing and Isotachophoresis : Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany /
B. J. Radola, D. Graesslin.
- Reprint 2019
- 1 online resource (624 p.)
Frontmatter -- Preface -- Contents -- Contributors -- Part I. Isoelectric Focusing -- 1. Theoretical and General Aspects -- Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / Stable pH Gradients - A Key Problem in Isoelectric Focusing / Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / Comparison of pH Gradients Used for Isoelectric Focusing / On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / 2. Carrier Ampholytes -- Carrier Ampholyte Distribution / Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / 3. Analytical Methodology -- Staining of Proteins in Polyacrylamide Gels / Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / pH Determinations in Isoelectric Focusing with an Iridium Electrode / Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / Does Lipoprotein Lipase Bind Ampholytes? / 4. Biochemical Applications -- Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / Fractionation of Nucleic Acids on Isoelectric Focusing / 5. Clinical Applications -- Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures / High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / 6. Two-Dimensional Techniques -- Biological Applications of Two-Dimensional Gel Electrophoresis / Plant Proteins Evaluated by Two-Dimensional Methods / Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / 7. Preparative Separations -- Isoelectric Focusing in Free Water / Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / Preparative Zone Convection Electrofocusing in Helical Glass Tubes / Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / 8. Separation of Cells -- New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / Part II. Isotachophoresis -- 1. Analytical and Preparative Methodology -- The Principle of Preparative Capillary Isotachophoresis / Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins / Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / 2. Applications -- Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / Index -- Backmatter Kolin, A. -- Rilbe, H. -- Chrambach, A. / Nguyen, Nga Y. -- Fawcett, J. S. -- Fredriksson, S. -- Brown, R. K. / Caspers, M. L. / Vinogradow, S. N. -- Radola, B. J. / Tschesche, H. / Schuricht, H. -- Grubhofer, N. / Borja, C. -- Vesterberg, O. / Hansén, L. -- Bispink, G. / Neuhoff, V. -- Zeineh, R. A. -- Zeineh, R. A. -- Leaback, D. H. -- Rosengren, Å. / Bjellqvist, B. / Gasparic, V. -- Gianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G. -- Delincée, H. / Radola, B. J. -- Bengtsson, G. / Olivecrona, T. -- Righetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C. -- Rücker, W. / Markotai, J. -- Narayanan, K. R. / Ray, A. S. -- Kubicz, A. / Wolanska, L. -- Drysdale, J. W. -- Allen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S. -- Arnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H. -- Jeppsson, J. O. -- Utermann, G. / Hess, M. / Vogelberg, K. H. -- Altland, K. -- Bours, J. -- Das, P. K. / Graesslin, D. / Goedde, H. W. -- Hamann, A. -- Wilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H. -- Hamberg, U. / Turpeinen, U. / Knuutinen, U. -- Pronk, J. C. -- Ivarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H. -- Stegemann, H. -- Kniifermann, H. -- Czempiel, W. -- Holmberg, K. / Wadström, T. -- Denckla, W. D. -- Winter, A. -- Wadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J. -- Quast, R. -- Sluyterman, L. A. A. E. / Wijdenes, J. -- Catsimpoolas, N. / Griffith, A. L. -- Just, W. W. / Werner, G. -- Manske, W. / Bohn, B. / Brossmer, R. -- Arlinger, L. -- Moberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H. -- Wielders, J. P. M. / Everaerts, F. M. -- Brogren, C. H. -- Delmotte, P. -- Bier, M. / Kopwillem, A. -- Peltre, G. / Brogren, C. H. -- Brogren, C. H. / Peltre, G. --
restricted access http://purl.org/coar/access_right/c_16ec
Mode of access: Internet via World Wide Web.
In German.
9783110070262 9783110873870
10.1515/9783110873870 doi
SCIENCE / General.
Frontmatter -- Preface -- Contents -- Contributors -- Part I. Isoelectric Focusing -- 1. Theoretical and General Aspects -- Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / Stable pH Gradients - A Key Problem in Isoelectric Focusing / Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / Comparison of pH Gradients Used for Isoelectric Focusing / On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / 2. Carrier Ampholytes -- Carrier Ampholyte Distribution / Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / 3. Analytical Methodology -- Staining of Proteins in Polyacrylamide Gels / Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / pH Determinations in Isoelectric Focusing with an Iridium Electrode / Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / Does Lipoprotein Lipase Bind Ampholytes? / 4. Biochemical Applications -- Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / Fractionation of Nucleic Acids on Isoelectric Focusing / 5. Clinical Applications -- Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures / High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / 6. Two-Dimensional Techniques -- Biological Applications of Two-Dimensional Gel Electrophoresis / Plant Proteins Evaluated by Two-Dimensional Methods / Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / 7. Preparative Separations -- Isoelectric Focusing in Free Water / Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / Preparative Zone Convection Electrofocusing in Helical Glass Tubes / Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / 8. Separation of Cells -- New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / Part II. Isotachophoresis -- 1. Analytical and Preparative Methodology -- The Principle of Preparative Capillary Isotachophoresis / Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins / Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / 2. Applications -- Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / Index -- Backmatter Kolin, A. -- Rilbe, H. -- Chrambach, A. / Nguyen, Nga Y. -- Fawcett, J. S. -- Fredriksson, S. -- Brown, R. K. / Caspers, M. L. / Vinogradow, S. N. -- Radola, B. J. / Tschesche, H. / Schuricht, H. -- Grubhofer, N. / Borja, C. -- Vesterberg, O. / Hansén, L. -- Bispink, G. / Neuhoff, V. -- Zeineh, R. A. -- Zeineh, R. A. -- Leaback, D. H. -- Rosengren, Å. / Bjellqvist, B. / Gasparic, V. -- Gianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G. -- Delincée, H. / Radola, B. J. -- Bengtsson, G. / Olivecrona, T. -- Righetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C. -- Rücker, W. / Markotai, J. -- Narayanan, K. R. / Ray, A. S. -- Kubicz, A. / Wolanska, L. -- Drysdale, J. W. -- Allen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S. -- Arnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H. -- Jeppsson, J. O. -- Utermann, G. / Hess, M. / Vogelberg, K. H. -- Altland, K. -- Bours, J. -- Das, P. K. / Graesslin, D. / Goedde, H. W. -- Hamann, A. -- Wilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H. -- Hamberg, U. / Turpeinen, U. / Knuutinen, U. -- Pronk, J. C. -- Ivarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H. -- Stegemann, H. -- Kniifermann, H. -- Czempiel, W. -- Holmberg, K. / Wadström, T. -- Denckla, W. D. -- Winter, A. -- Wadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J. -- Quast, R. -- Sluyterman, L. A. A. E. / Wijdenes, J. -- Catsimpoolas, N. / Griffith, A. L. -- Just, W. W. / Werner, G. -- Manske, W. / Bohn, B. / Brossmer, R. -- Arlinger, L. -- Moberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H. -- Wielders, J. P. M. / Everaerts, F. M. -- Brogren, C. H. -- Delmotte, P. -- Bier, M. / Kopwillem, A. -- Peltre, G. / Brogren, C. H. -- Brogren, C. H. / Peltre, G. --
restricted access http://purl.org/coar/access_right/c_16ec
Mode of access: Internet via World Wide Web.
In German.
9783110070262 9783110873870
10.1515/9783110873870 doi
SCIENCE / General.