TY  - BOOK
AU  - Graesslin,D.
AU  - Radola,B.J.
TI  - Electrofocusing and Isotachophoresis: Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany 
SN  - 9783110070262
PY  - 2019///]
CY  - Berlin, Boston : 
PB  - De Gruyter, 
KW  - SCIENCE / General
KW  - bisacsh
N1  - Frontmatter --; Preface --; Contents --; Contributors --; Part I. Isoelectric Focusing --; 1. Theoretical and General Aspects --; Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea /; Kolin, A. --; Stable pH Gradients - A Key Problem in Isoelectric Focusing /; Rilbe, H. --; Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential /; Chrambach, A. / Nguyen, Nga Y. --; Comparison of pH Gradients Used for Isoelectric Focusing /; Fawcett, J. S. --; On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing /; Fredriksson, S. --; 2. Carrier Ampholytes --; Carrier Ampholyte Distribution /; Brown, R. K. / Caspers, M. L. / Vinogradow, S. N. --; Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods /; Radola, B. J. / Tschesche, H. / Schuricht, H. --; Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range /; Grubhofer, N. / Borja, C. --; 3. Analytical Methodology --; Staining of Proteins in Polyacrylamide Gels /; Vesterberg, O. / Hansén, L. --; Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF /; Bispink, G. / Neuhoff, V. --; Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing /; Zeineh, R. A. --; Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry /; Zeineh, R. A. --; Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins /; Leaback, D. H. --; A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis /; Rosengren, Å. / Bjellqvist, B. / Gasparic, V. --; pH Determinations in Isoelectric Focusing with an Iridium Electrode /; Gianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G. --; Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing /; Delincée, H. / Radola, B. J. --; Does Lipoprotein Lipase Bind Ampholytes? /; Bengtsson, G. / Olivecrona, T. --; 4. Biochemical Applications --; Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation /; Righetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C. --; Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems /; Rücker, W. / Markotai, J. --; A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing /; Narayanan, K. R. / Ray, A. S. --; Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) /; Kubicz, A. / Wolanska, L. --; Fractionation of Nucleic Acids on Isoelectric Focusing /; Drysdale, J. W. --; 5. Clinical Applications --; Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies /; Allen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S. --; Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures /; Arnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H. --; High Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing /; Jeppsson, J. O. --; Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation /; Utermann, G. / Hess, M. / Vogelberg, K. H. --; Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing /; Altland, K. --; The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing /; Bours, J. --; Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality /; Das, P. K. / Graesslin, D. / Goedde, H. W. --; Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing /; Hamann, A. --; Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay /; Wilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H. --; Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing /; Hamberg, U. / Turpeinen, U. / Knuutinen, U. --; A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing /; Pronk, J. C. --; 6. Two-Dimensional Techniques --; Biological Applications of Two-Dimensional Gel Electrophoresis /; Ivarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H. --; Plant Proteins Evaluated by Two-Dimensional Methods /; Stegemann, H. --; Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis /; Kniifermann, H. --; Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins /; Czempiel, W. --; Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens /; Holmberg, K. / Wadström, T. --; 7. Preparative Separations --; Isoelectric Focusing in Free Water /; Denckla, W. D. --; Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects /; Winter, A. --; Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis /; Wadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J. --; Preparative Zone Convection Electrofocusing in Helical Glass Tubes /; Quast, R. --; Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential /; Sluyterman, L. A. A. E. / Wijdenes, J. --; 8. Separation of Cells --; New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques /; Catsimpoolas, N. / Griffith, A. L. --; Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction /; Just, W. W. / Werner, G. --; Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient /; Manske, W. / Bohn, B. / Brossmer, R. --; Part II. Isotachophoresis --; 1. Analytical and Preparative Methodology --; The Principle of Preparative Capillary Isotachophoresis /; Arlinger, L. --; Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins /; Moberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H. --; Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis /; Wielders, J. P. M. / Everaerts, F. M. --; Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing /; Brogren, C. H. --; Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization /; Delmotte, P. --; 2. Applications --; Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers /; Bier, M. / Kopwillem, A. --; Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis /; Peltre, G. / Brogren, C. H. --; Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies /; Brogren, C. H. / Peltre, G. --; Index --; Backmatter; restricted access
UR  - https://doi.org/10.1515/9783110873870
UR  - https://www.degruyter.com/cover/covers/9783110873870.jpg
ER  -