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001 248171
003 IT-RoAPU
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008 190723s2019 gw fo d z ger d
020 _a9783110070262
_qprint
020 _a9783110873870
_qPDF
024 7 _a10.1515/9783110873870
_2doi
035 _a(DE-B1597)9783110873870
035 _a(DE-B1597)39933
035 _a(OCoLC)1110712791
040 _aDE-B1597
_beng
_cDE-B1597
_erda
072 7 _aSCI000000
_2bisacsh
084 _aonline - DeGruyter
245 0 0 _aElectrofocusing and Isotachophoresis :
_bProceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany /
_cB. J. Radola, D. Graesslin.
250 _aReprint 2019
264 1 _aBerlin ;
_aBoston :
_bDe Gruyter,
_c[2019]
264 4 _c©1977
300 _a1 online resource (624 p.)
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 0 _tFrontmatter --
_tPreface --
_tContents --
_tContributors --
_tPart I. Isoelectric Focusing --
_t1. Theoretical and General Aspects --
_tReminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea /
_rKolin, A. --
_tStable pH Gradients - A Key Problem in Isoelectric Focusing /
_rRilbe, H. --
_tElectrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential /
_rChrambach, A. / Nguyen, Nga Y. --
_tComparison of pH Gradients Used for Isoelectric Focusing /
_rFawcett, J. S. --
_tOn the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing /
_rFredriksson, S. --
_t2. Carrier Ampholytes --
_tCarrier Ampholyte Distribution /
_rBrown, R. K. / Caspers, M. L. / Vinogradow, S. N. --
_tCharacterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods /
_rRadola, B. J. / Tschesche, H. / Schuricht, H. --
_tSynthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range /
_rGrubhofer, N. / Borja, C. --
_t3. Analytical Methodology --
_tStaining of Proteins in Polyacrylamide Gels /
_rVesterberg, O. / Hansén, L. --
_tIsoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF /
_rBispink, G. / Neuhoff, V. --
_tSoft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing /
_rZeineh, R. A. --
_tImmunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry /
_rZeineh, R. A. --
_tRapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins /
_rLeaback, D. H. --
_tA Simple Method of Choosing Optimum pH-Conditions for Electrophoresis /
_rRosengren, Å. / Bjellqvist, B. / Gasparic, V. --
_tpH Determinations in Isoelectric Focusing with an Iridium Electrode /
_rGianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G. --
_tAnomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing /
_rDelincée, H. / Radola, B. J. --
_tDoes Lipoprotein Lipase Bind Ampholytes? /
_rBengtsson, G. / Olivecrona, T. --
_t4. Biochemical Applications --
_tZein: Macromolecular Properties, Biosynthesis and Genetic Regulation /
_rRighetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C. --
_tGrowth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems /
_rRücker, W. / Markotai, J. --
_tA Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing /
_rNarayanan, K. R. / Ray, A. S. --
_tPhylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) /
_rKubicz, A. / Wolanska, L. --
_tFractionation of Nucleic Acids on Isoelectric Focusing /
_rDrysdale, J. W. --
_t5. Clinical Applications --
_tPolyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies /
_rAllen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S. --
_tPolyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α1 Pi) Phenotyping: II. Immunochemical Procedures /
_rArnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H. --
_tHigh Resolution Phenotyping of α1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing /
_rJeppsson, J. O. --
_tBroad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation /
_rUtermann, G. / Hess, M. / Vogelberg, K. H. --
_tScreening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing /
_rAltland, K. --
_tThe Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing /
_rBours, J. --
_tSeparation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality /
_rDas, P. K. / Graesslin, D. / Goedde, H. W. --
_tMicroheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing /
_rHamann, A. --
_tDiagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay /
_rWilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H. --
_tMicroheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing /
_rHamberg, U. / Turpeinen, U. / Knuutinen, U. --
_tA Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing /
_rPronk, J. C. --
_t6. Two-Dimensional Techniques --
_tBiological Applications of Two-Dimensional Gel Electrophoresis /
_rIvarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H. --
_tPlant Proteins Evaluated by Two-Dimensional Methods /
_rStegemann, H. --
_tTwo-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis /
_rKniifermann, H. --
_tMammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins /
_rCzempiel, W. --
_tCrossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens /
_rHolmberg, K. / Wadström, T. --
_t7. Preparative Separations --
_tIsoelectric Focusing in Free Water /
_rDenckla, W. D. --
_tPreparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects /
_rWinter, A. --
_tPreparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis /
_rWadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J. --
_tPreparative Zone Convection Electrofocusing in Helical Glass Tubes /
_rQuast, R. --
_tChromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential /
_rSluyterman, L. A. A. E. / Wijdenes, J. --
_t8. Separation of Cells --
_tNew Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques /
_rCatsimpoolas, N. / Griffith, A. L. --
_tContinuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction /
_rJust, W. W. / Werner, G. --
_tPreparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient /
_rManske, W. / Bohn, B. / Brossmer, R. --
_tPart II. Isotachophoresis --
_t1. Analytical and Preparative Methodology --
_tThe Principle of Preparative Capillary Isotachophoresis /
_rArlinger, L. --
_tPreparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins /
_rMoberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H. --
_tQuantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis /
_rWielders, J. P. M. / Everaerts, F. M. --
_tThin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing /
_rBrogren, C. H. --
_tIsotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization /
_rDelmotte, P. --
_t2. Applications --
_tPlasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers /
_rBier, M. / Kopwillem, A. --
_tImmuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis /
_rPeltre, G. / Brogren, C. H. --
_tAnalytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies /
_rBrogren, C. H. / Peltre, G. --
_tIndex --
_tBackmatter
506 0 _arestricted access
_uhttp://purl.org/coar/access_right/c_16ec
_fonline access with authorization
_2star
538 _aMode of access: Internet via World Wide Web.
546 _aIn German.
588 0 _aDescription based on online resource; title from PDF title page (publisher's Web site, viewed 23. Jul 2019)
650 7 _aSCIENCE / General.
_2bisacsh
700 1 _aGraesslin, D.
_ecuratore
700 1 _aRadola, B. J.
_ecuratore
850 _aIT-RoAPU
856 4 0 _uhttps://doi.org/10.1515/9783110873870
856 4 2 _3Cover
_uhttps://www.degruyter.com/cover/covers/9783110873870.jpg
942 _cEB
999 _c248171
_d248171